Method of making aspartic acid and purifying aspartase

Details Method of making aspartic acid and purifying aspartase Method of making aspartic acid and purifying aspartase

1984-01-19

1986-02-11

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

435188, 435232, 435814, 435839, C12P 1320, C12N 996, C12N 988, C12R 1125

Patent

active

045699119

ABSTRACT:
The aspartase from a mutant Bacillus subtilis, NRRL B-15536, is produced in relatively high cell yield within a comparatively short time. The enzyme converts fumaric acid to L-aspartic acid stoichiometrically with outstanding selectivity and productivity. The enzyme is stabilized by divalent magnesium ions, 2-mercaptoethanol, and ammonium furmarate, and can be conveniently purified with high recovery.

REFERENCES:
patent: 3214345 (1965-10-01), Chibata et al.
patent: 3791926 (1974-02-01), Chibata et al.
patent: 4141857 (1979-02-01), Levy et al.
patent: 4391910 (1983-07-01), Kimura et al.
Agricultural and Biological Chemistry, vol. 29, No. 7, pp. 665-671, (1965).

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