Method of making an electrophoresis medium

Details Method of making an electrophoresis medium Method of making an electrophoresis medium

1988-08-19

1989-05-30

Niebling, John F.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, 2041801, G01N 2726

Patent

active

048348546

ABSTRACT:
A method of making an electrophoresis medium having a predetermined gradient in concentrations of an acrylamide compound and a cross-linking agent comprises the steps of mixing two kinds of aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations such that a mixing ratio of the aqueous solutions to each other is changed gradually, thereby to form a predetermined gradual change (gradient) in concentrations of the acrylamide compound and the cross-linking agent in the mixture, and carrying out cross-linking polymerization of the mixture in the presence of a polymerization initiator. Viscosity values of the two kinds of the aqueous solutions containing the acrylamide compound and the cross-linking agent in different concentrations prior to the mixing are adjusted to be substantially equal to each other.

REFERENCES:
patent: 3443520 (1969-05-01), Nejame, Jr.
patent: 4620794 (1986-11-01), Leka
patent: 4704198 (1987-11-01), Ebersole et al.

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