Method of making an assay compound

Details Method of making an assay compound Method of making an assay compound

1995-05-18

1998-03-31

Gitomer, Ralph

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 29, 435968, C12Q 100, C12Q 102

Patent

active

057337195

ABSTRACT:
An assay compound or a salt thereof for assaying the activity of an enzyme inside a metabolically active whole cell is disclosed. The assay compound includes a leaving group and an indicator group. The leaving group is selected from the group comprising amino acids, peptides, saccharides, sulfates, phosphates, esters, phosphate esters, nucleotides, polynucleotides, nucleic acids, pyrimidines, purines, nucleosides, lipids and mixtures thereof. The indicator group is selected from compounds which have a first state when joined to the leaving group, and a second state when the leaving group is cleaved from the indicator group by the enzyme. Preferably, the indicator compounds are rhodamine 110, rhodol, and fluorescein and analogs of these compounds. A method of synthesizing the compound as well as methods of using these compounds to measure enzyme activity are also disclosed.

REFERENCES:
patent: 3786140 (1974-01-01), Meyer-Bertenrath
patent: 4275153 (1981-06-01), Gargiulo et al.
patent: 4279810 (1981-07-01), Claeson et al.
patent: 4336186 (1982-06-01), Gargiulo et al.
patent: 4557862 (1985-12-01), Mangel et al.
patent: 4640893 (1987-02-01), Mangel et al.
patent: 5070012 (1991-12-01), Nolan et al.
patent: 5208148 (1993-05-01), Haugland et al.
patent: 5290682 (1994-03-01), Meneghini et al.
patent: 5330889 (1994-07-01), Monget
patent: 5443986 (1995-08-01), Haughland et al.
Mitchell, G., Novel Rhodamine Tripeptide Substrate for Manual and Automated . . . Time Test, Thrombosis Research, vol. 40, 339-349, 1985.
Hegazi, F., Factors Affecting the Caseinolytic Activity of Lactobacillus plantarum, Die Nahrung, vol. 31, No. 3, 199-206, 1987.
Watson, Cytometry, vol. 1, No. 2, pp. 143-151 (1980).
Leytus et al, Biochem. J., vol. 209, pp. 299-307 (1983).
Leytus et al, Biochimica et Biophysica Acta, vol. 788, pp. 74-86 (1984).
Melhado et al, J. Am. Chem. Soc., vol. 104, pp. 7299-7306 (1982).
Leytus et al, Biochem. J., vol. 215, pp. 253-260 (1983).
Kanaoka et al, Chem. Pharm. Bull, vol. 25, No. 2, pp. 362-363 (1977).
Morita et al, J. Biochem., vol. 82, pp. 1495-1498 (1977).
Mononen et al, Clinical Chemistry, vol. 40, No. 3, pp. 385-388 (1994).
Livingston et al, Biochemistry, vol. 20, No. 15, pp. 4298-4306 (1981).
Rothe et al, Biol. Chem. Hoppe-Seyler, vol. 373, pp. 547-554 (1992).
Mangel et al, Nature, vol. 361, pp. 274-275 (Jan. 21, 1993).
Huang et al, The Journal of Histochemistry and Cytochemistry, vol. 41, No. 2, pp. 313-317 (1993).
Lottenberg et al, Methods in Enzymology, vol. 80, pp. 341-361 (1981).
Saifuku et al, Clinica Chimica Acta, vol. 84, No. 1/2, pp. 85-91 (1978).
Dive et al, Cytometry, vol. 8, pp. 552-561 (1987).
BioProbes 21--New Products and Applications from Molecular Probes, Inc., cover page, contents page and pp. 18-21 (Nov. 1994).
Duffy et al, Clinical Chemistry, vol. 38, No. 10, pp. 2114-2116 (1992).
Valet et al, Ann. NY Acad. Sci., vol. 677, pp. 233-251 (1993).
Cox et al, Cytometry, vol. 8, pp. 267-272 (1987).
Hjorth, Brain Topography, vol. 2, Nos. 1/2, pp. 57-61 (1989).
Windham et al, Journal of Computer Assisted Tomography, vol. 12, No. 1, pp. 1-9 (1988).

Affiliated with

Also associated with

Rating

Method of making an assay compound does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of making an assay compound, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of making an assay compound will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-50662