Method of making a polysaccharide vaccine

Details Method of making a polysaccharide vaccine Method of making a polysaccharide vaccine

1981-10-29

1982-10-26

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435885, C12P 1904

Patent

active

043562639

ABSTRACT:
Culturing Streptococcus agalactiae in a nutrient broth containing at least 10 g/l of glucose while maintaining the pH at 6-8 by the addition of alkaline material produces polysaccharide antigen including a fraction having a molecular size from 8-11.times.10.sup.5 d. By employing a broth free from constituents having a molecular size above 10.sup.5, the antigens can be separated from the broth in high yield after harvesting the organisms. Vaccination of humans with Streptococcus agalactiae polysaccharide antigens having molecular sizes above 5.times.10.sup.5 produces in selected individuals specific antibody levels above 200 .mu.g/ml of antisera; globulins fractionated from such antisera contain most of the antibody and can be used for passive immunization.

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