Method of making a genetically modified mammalian cell

Details Method of making a genetically modified mammalian cell Method of making a genetically modified mammalian cell

2006-11-02

2010-06-22

Crouch, Deborah (Department: 1632)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

C435S440000, C435S455000, C435S354000

Reexamination Certificate

active

07741079

ABSTRACT:
A method of making a genetically modified mammalian cell, the method including selecting a first codon of a parent polynucleotide that encodes a polypeptide for replacement with a synonymous codon, wherein the synonymous codon is selected on the basis that it exhibits a higher translational efficiency in a first type of mammalian cell than the first codon in a comparison of translational efficiencies of codons in cells of the first type, replacing the first codon with the synonymous codon to form a synthetic polynucleotide, and introducing the synthetic polynucleotide into a mammalian cell to produce the genetically modified mammalian cell.

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