Method of making a cloning vector

Details Method of making a cloning vector Method of making a cloning vector

1982-03-12

1983-11-15

Tanenholtz, Alvin E.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

C12N 1500

Patent

active

044156600

ABSTRACT:
A cloning vector useful in recombining DNA (i.e. recombinant DNA studies) is made by mutagenesis of a satellite bacteriophage P4 wt or P4 vir.sub.1 and subsequent separation and purification of the mutant progeny in a cesium chloride equilibrium density gradient. On such a cesium chloride gradient the mutant is found in a range of densities from 1.35 to 1.42 g/ml at 24.degree. C. with peaks at about 1.42, 1.39, and 1.35 g/ml.

REFERENCES:
Gibbs et al., Virology, vol. 53, pp. 24-39 (1973).
Lamanna et al., The Williams & Wilkins Co., pp. 723-727 (1965).
Metzler, Biochemistry, The Chemical Reactions of Living Cells, pp. 945-946.
Garland et al., Biochemistry of Genetic Engineering (1979).

Affiliated with

Also associated with

Rating

Method of making a cloning vector does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of making a cloning vector, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of making a cloning vector will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-159947