Method of maintaining or improving a nitrile hydratase activity

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing nitrogen-containing organic compound

Reexamination Certificate

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C435S232000, C435S252330

Reexamination Certificate

active

10850374

ABSTRACT:
The object of this invention is to provide a method which not only maintains the nitrile hydratase activity of a nitrile hydratase-containing cell or a treated material of the cell under conditions where the cell does not grow, but also improves the nitrile hydratase activity of a nitrile hydratase-containing cell or a treated material of the cell whose activity was once reduced. This invention relates to a method of maintaining or improving a nitrile hydratase activity which comprises bringing a nitrile hydratase-containing cell or a treated material of the cell into contact with an oxidizing agent under conditions where the cell does not grow, as well as a method of producing an amide compound from a nitrile compound, which comprises using the cell brought into contact with an oxidizing agent or a treated material of the cell.

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Search Report dated Aug. 4, 2005 in corresponding French Application No. 0405712000.

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