Method of magnetic separation of cells and the like, and microsp

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210222, G01N 3100, G01N 2146

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active

042306850

ABSTRACT:
Magneticaly-responsive microspheres having Protein A associated with the outer surfaces thereof are reacted with antibodies selective to the cells, bacteria, or viruses to be separated from a mixed population to attach the antibodies in oriented relation with their Fab arms extending outwardly, and the microspheres are then used in a magnetic separation procedure. The preferred microspheres are prepared from a mixture of albumin, Protein A, and magnetic particles, the microspheres being prepared so that the Protein A is present in the exterior surfaces for antibody binding.

REFERENCES:
patent: 3970518 (1976-07-01), Giaever
patent: 4018886 (1977-04-01), Giaever
Kessler-Dissertation Abst. Int(B), 1976, vol. 37, No. 3, pp. 1172-1173.

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