Method of lyophilization of mammalian sperm cells

Details Method of lyophilization of mammalian sperm cells Method of lyophilization of mammalian sperm cells

1991-02-15

1998-12-15

Saucier, Sandra E.

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

424561, A01N 102, A61K 3552

Patent

active

058494731

ABSTRACT:
A method is provided for the lyophilization of mammalian sperm cells which can be stored and reconstituted to provide morphologically intact cells. The method is advantageous in that the sperm cells may be stored at ambient temperatures for extended periods of time and recovered morphologically intact with DNA-containing heads and intact flagella. The lyophilization medium comprises a carbohydrate and a polymer selected from PVP, HES, dextran and poloxamers or mixtures thereof.

REFERENCES:
patent: 4874690 (1989-10-01), Goodrich et al.
Grant and Hacth's Chemical Dictionary p.364 (1987).
Singh et al Indian J. Vet. Sci 37: 1-7 (1967).
Watanabe et al J. Fac. Fish. Anim. Husb. 11:33-37 (1972).

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