Method of labelling leucocytes with Tc-99m, d,1-HMPAO

Details Method of labelling leucocytes with Tc-99m, d,1-HMPAO Method of labelling leucocytes with Tc-99m, d,1-HMPAO

1989-07-20

1994-01-18

Alexander, Lyle A.

Chemistry: analytical and immunological testing

Including use of radioactive properties

436177, 435 724, 252645, G01N 2300, G01N 118, C09K 1100

Patent

active

052799689

ABSTRACT:
An improved method for radio-labelling leucocytes in vitro with Tc-99m d,1-HMPAO (Technetium-99m-d,1-hexamethyl propyleneamine oxime) for use in scintigraphic imaging of inflammatory lesions enhances the labelling efficiency by incubating the leucocytes with Tc-99m d,1-HMPAO in the presence of an acid citrate dextrose solution and by depleting residual RBCs from the leucocytes prior to such incubation step. The RBC depletion is accomplished by means of a rocking step.

REFERENCES:
patent: 3725295 (1973-04-01), Eckelman et al.
patent: 4311688 (1982-01-01), Burchiel et al.
patent: 4434237 (1984-02-01), Dinarello
patent: 4443426 (1984-04-01), Thakur
patent: 4452774 (1984-06-01), Jones et al.
patent: 4455379 (1984-06-01), Bekesi et al.
patent: 4456690 (1984-06-01), Cais et al.
patent: 4666834 (1987-05-01), Bekesi et al.
H. J. Danpure et al., Nuclear Medicine Communications, (1988) vol. 9, pp. 681-685.
A. M. Peters et al., Lancet (1986) vol. 2 pp. 946-949.
Joseph C. Hung et al., Journal of Nuclear Medicine (1988) vol. 29, pp. 1568-1576.
N. N. Solanki et al., Nuclear Medicine Communications (1988) vol. 9, pp. 753-761.
Operating Instructions for the Hema-Tek Aliquot Mixer from Miles Laboratories, Inc.
Appendex "A": Statement regarding a public use of a Rocker Arm.

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