Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2007-04-20
2010-02-09
Lucas, Zachariah (Department: 1648)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S350000, C530S412000, C435S183000, C435S213000
Reexamination Certificate
active
07659380
ABSTRACT:
The present invention provides methods of isolation and purification ofStreptomyces griseustrypsin (SGT) from PRONASE protease mixture in a single affinity chromatography step and uses of the purified SGT.
REFERENCES:
patent: 4973554 (1990-11-01), Luong et al.
Ellquali et al., Affinity of trypsin for Amidine Derivatives Immobilized on Dextran-Coated Silica Supports, Chromsymp, 2215, p. 255-265 (1991).
Hatanaka et al., “Interactions of derivatives of guanidinophenylalanine and guanidinophenylglycine with Streptomyces griseus trypsin,” Biochemica et Biophysica Acta, vol. 832, pp. 274-279 (1985).
Koo et al., “Purification and characterization of Streptomyces griseusStreptomyces griseus trypsin overexpressed in Streptomyces lividans, ” Journal of Microbiology and Biotechnology, vol. 8(4), pp. 333-340 (1998).
Shimura et al., Affinophoresis of Trypsins, Journal of Biochem, vol. 92, p. 1615-1622 (1982).
Mitterer Artur
Mundt Wolfgang
Reiter Manfred
Tauer Christa
Baxter Healthcare S.A.
Lucas Zachariah
Townsend and Townsend / and Crew LLP
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