Method of isolating restriction fragment deletions in vibrio cho

Details Method of isolating restriction fragment deletions in vibrio cho Method of isolating restriction fragment deletions in vibrio cho

1984-02-17

1992-08-04

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4351721, 4352523, 435909, 4353201, 935 56, 935 72, C12N 1500, C12N 120, C12R 163

Patent

active

051358620

ABSTRACT:
This invention relates to a method of isolating deletion mutants of Vibrio cholerae, wherein the deletion is predetermined by digestion with restriction endonucleases of known specificity. The deletions are inserted into the Vibrio cholerae chromosome by in vivo recombination between a plasmid carrying the desired deletion, with adjacent flanking sequences, and the Vibrio cholerae chromosome. The invention includes the isolation and characterization of a new Vibrio cholerae strain having a deletion in the tox gene, as defined by Acc I, Xba I, Cla I and/or Hind III restriction endonuclease sites.

REFERENCES:
patent: 4882278 (1989-11-01), McKalanos
patent: 4935364 (1990-06-01), Kaper et al.
Sigel, S et al., Infection & Immunity, vol. 32, pp. 474-479, 1981.
Honda, T. et a., Proc. Natl Acad Sci., vol. 76, pp. 2052-2056, 1979.
Mekalanos, J. et al., Nature, vol. 306, pp. 551-557, Dec. 1983.
Kaper, J. et al., Vaccines 85-Molecular and Chemical Basis of Resistance to Viral, Bacterial and Parasitic Diseases (Cold Spring Harbor, N.Y. pp. 107-111, 1985.
Levine, M. et al., Transactions Royal Society of Tropical Medicine and Hygiene, vol. 73, pp. 3-9, 1979.
Curlin, G. et al., Proc. 11th Joint Conf. on Cholera U.S.--Japan Cooperative Med. Sci. Program, pp. 314-329, 1975.
Noriki, H., Proc. 12th Joint Conf on Cholera U.S.--Japan Cooperative Med Sci Program, pp. 302-310, 1977.
Levine, M. et al., Microbiol Reviews, pp. 510-550, Dec. 1983.
Mekalanos, J. et al, Proc Natl Acad Sci, vol. 79, pp. 151-155, 1982.
Pearson, G. D., et al., Proc Natl Acad Sci., vol. 79 pp. 2976-2980, 1982.
Cash, R. A. et al. J. Infect. Diseases, vol. 129(1) pp. 45-52, 1974.
Matzke, A. J. et al. J. Molec & App Genetics, vol. pp. 39-49, 1981.
Chilton et al., Stadler Symp, vol. 13, pp. 39-52, 1981.
Sutcliffe, J. G. et al. Genetic Engineering (Charabarty, A. Ed) pp. 83-101, 1978.
Kaper, J. et al., Nature, vol. 308, pp. 655-658, Apr. 12, 1984.
Lockman, H. et al., J. Biol Chem, vol. 258(22) pp. 13722-13726, 1983.
Sublett, R. D. et al. Inf & Imm., vol. 32(3) pp. 1132-1138, 1981.
Thompson, J. et al. J. Bact., vol. 148, pp. 374-378, 1981.
Kaper, J. B., et al., Inf & Imm., vol. 32(2) pp. 661-667, 1981.
Levine, M. M., Acute Enteric Infections in Children, New Prospects for Treatment, (Holme et al Eds) pp. 443-459, 1981.
Levine et al., Lancet, pp. 467-470 Aug. 1988).
Levine et al., Infection and Immunity, pp. 161-167 (Jan. 1988).
Brickman, Abstract of the Annual Meeting (1989). American Society for Microbiology.
Brickman, et al. Infection and Immunity, vol. 58, No. 12, pp. 4142-4144 (Dec. 1990).
Levine et al., Infection and Immunity vol. 43, No. 2 pp. 515-522 (Feb. 1984).
Mekalanos, Cell, vol. 35, pp. 252-263 (Nov. 1983).
Sanchez and Holmgren, PNAS, vol. 86, pp. 481-485 (Jan. 1989).
Herrington et al., J. Exp. Med. vol. 168, pp. 1487-1492 (Oct. 1988).
Cryz et al., Vaccine, vol. 8, pp. 577-580 (Dec. 1990).
76th Congress--1st Session, House of Representatives Report No. 970, Jun. 28, 1939, two pages (1a and 2a).
A.-M. Svennerholm et al., The Lancet, pp. 305-308 (Feb. 6, 1982).
Black, Robert E., manuscript "Protective Efficacy in Man of Killed Whole Vibrio Oral Cholera Vaccine With and Without The B Subunit of Cholera Toxin" to be published in Infection and Immunity.
Holmgren, J., Nature, vol. 292, pp. 413-417 (1981).
Levine, Myron M. et al., Microbiological Reviews, vol. 47, pp. 510-550 (1983).
Svennerholm, A.--M. et al., Bulletin of the World Health Organization, vol. 62, pp. 909-918 (1984).
Cloned Cholera Enterotoxin Genes in Study and Prevention of Cholera, Microbiology Abstracts, J. B. Kaper et al., Part B, vol. 17, No. 8, Aug. 1982, p. 46.
No. 7654-J17, CAS Data Base Services, Bethesda, MD US; and Lancet, vol. 2, No. 8256, Nov. 1981, pp. 1162-1163.
The Expression of Biologically Active Cholera Toxin in Escherichia coli; M. L. Gennaro et al.; Chemical Abstracts, vol. 97, No. 10, Oct. 25, 1982, pp. 194, 195, No. 139632d, Columbus, OH, U.S. and Nucleic Acids Res. 1982, 10(16), 4883-4890.
Molecular Cloning of Vibrio cholerae Enterotoxin Genes in Escherichia coli K-12, G. D. N. Perason et al; Proceedings of the National Academy of the United States of America, vol. 79, No. 9, May 1982, pp. 2976-2980, Baltimore, U.S.
Isolation of enterotoxin Structural Gene Deletion Mutations in Vibrio cholerae Induced by Two Mutagenic Vibriophages, J. J. Mekalanos et al., Proceedings of the National Academy of Sciences of the United States of America, vol. 79, No. 1, Jan. 1982, pp. 151-155, Baltimore, MD, U.S.
Cholera toxin genes: Nucleotide Sequence, Deletion Analysis and Vaccine Development, Nature, vol 306, No. 5943, Dec. 8, 1983, pp. 551-557, Chesham, Bucks, GB; J. J. Mekalanos et al.

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