Method of isolating polysaccharide producing bacteria from non-p

Details Method of isolating polysaccharide producing bacteria from non-p Method of isolating polysaccharide producing bacteria from non-p

1992-03-26

1994-08-16

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435100, 435101, 435170, 4352524, 4352536, 435261, 435874, C12P 1912, C12P 1904, C12N 102, C12N 120

Patent

active

053386817

ABSTRACT:
The invention relates to a method for isolating a polysaccharide extracellularly producing bacteria from a mixed culture of non-polysaccharide and polysaccharide producing bacteria, The method uses a streaking technique on a nutrient medium containing unsubstituted gellan as a gellant. The polysaccharide extracellularly producing bacteria are isolated by the act of non-polysaccharide extracellularly producing bacteria sinking into the nutrient medium. However, the polysaccharide extracellularly producing bacteria do not sink into the nutrient medium. The isolated cells are capable of producing an exopolysaccharide such as P4. Further the isolated cells are capable of producing gellan. The specific polysaccharide producing bacteria are of the genera Auromonas, Pseudomonas and Sphingomonas.

REFERENCES:
patent: 4535153 (1985-08-01), Kang et al.
patent: 4567140 (1986-01-01), Voelskow et al.
patent: 5071976 (1991-12-01), Stirling
patent: 5093253 (1992-08-01), Nolan
patent: 5125099 (1992-06-01), Cornish et al.
patent: 5130249 (1992-07-01), Hardin et al.

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