Method of isolating monoclonal antibodies from hybridoma culture

Details Method of isolating monoclonal antibodies from hybridoma culture Method of isolating monoclonal antibodies from hybridoma culture

1984-05-08

1985-08-06

Kight, John

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

435 68, 435240, 435241, C07G 700, C07G 7028

Patent

active

045334960

ABSTRACT:
Monoclonal antibodies are isolated from spent culture medium of the in vitro growth of hybridoma cells by treating said spent cell culture medium or a concentrate thereof with a water-insoluble, cross-linked polyelectrolyte copolymer.

REFERENCES:
patent: 3555001 (1971-01-01), Wallis et al.
patent: 4097473 (1978-06-01), Lewis et al.
patent: 4382028 (1983-05-01), Paget
Reisfeld et al., Production and Characterization . . . Antigens, Hybridoma in Cancer Diagnosis and Treatment, (21), 1982 Raven Press, N.Y., (Ed., Mitchell et al.), p. 183.
Monoclonals Disappoint . . . Clinical Trials, Newsweek, May 1984, p. 8.
Bumal et al., Unique Glycoprotein . . . Human Melanoma Cells, PNAS, (vol. 79), 1982, pp. 1245-1249.
Bumal et al., Monoclonal Antibody . . . in vivo Tumor Growth, PNAS, (vol. 80), 1983, pp. 529-533.
Monoclonal Antibodies, Milstein, pp. 66-74.

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