Method of isolating blood-clotting factor IX

Details Method of isolating blood-clotting factor IX Method of isolating blood-clotting factor IX

1990-05-01

1991-10-29

Draper, Garnette D.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Blood proteins or globulins, e.g., proteoglycans, platelet...

530384, 530416, 530413, C07K 302, C07K 320, C07K 322

Patent

active

050617893

ABSTRACT:
A method of isolating blood-clotting Factor IX. A solution containing the Factor IX is adsorbed onto an adsorbent carrying .alpha.-hydroxylamine groups, and the factor is eluted. The Factor IX from the eluate is adsorbed onto a matrix carrying sulfated carbohydrates, with the conductivity adjusted to 13-17 mS/cm, and eluted with a salt gradient. The pure Factor IX is concentrated and freeze-dried.

REFERENCES:
patent: 3920625 (1975-11-01), Anderson et al.
patent: 4721572 (1988-01-01), Jordan
patent: 4725673 (1988-02-01), Herring
Matsumoto et al., (1980), J. Biochem. 87, 535-540.
C. Michalski et al., "Large-Scale Production and Properties of a Solvent-Detergent . . . ", Vox Sang 55 (1988) 4, pp. 202-210.
T. Burnouf et al., "Therapeutic Advantage of a High-Purity Factor IX . . . ", Int. Symp. on Biotechnology of Plasma Proteins, Abstr. 4.09), (1988).

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