Method of isolating antibiotic biosynthetic genes

Details Method of isolating antibiotic biosynthetic genes Method of isolating antibiotic biosynthetic genes

1985-06-07

1990-06-19

Martinell, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 4351723, 43525235, 536 27, 935 11, 935 14, 935 16, 935 75, 935 78, 935 79, 935 80, C12Q 168, C12P 1934, C12N 1500, C12N 120, C07H 2104

Patent

active

049353403

ABSTRACT:
The present invention is a method for isolating antibiotic biosynthetic genes. To practice the method, an antibiotic resistance-conferring DNA segment is labelled and used as a probe to find, via DNA hydridization, homologous DNA in a genetic library which comprises chromosomal and plasmid DNA of an antibiotic-producing organism. Individual vectors of the genetic library which hybridize to the antibiotic resistance-conferring gene, and which comprise .about.1-45 kb of contiguous DNA from the antibiotic-producing organism, which also comprise an antibiotic biosynthetic gene. The present method is exemplified by using the erythromycin resistance-conferring gene of Streptomyces erythreus to clone the erythromycin biosynthetic pathway from the same organism. The erythromycin biosynthetic pathway isolated with the present method synthesizes erythromycin when introduced into S. lividans TK23.

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