Method of isolating and purifying nucleic acids from biological

Details Method of isolating and purifying nucleic acids from biological Method of isolating and purifying nucleic acids from biological

1986-12-01

1990-06-19

Yarbrough, Amelia B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 435803, 436 17, 536 27, 935 1, 935 19, 935 20, 935 21, 935 9, 935 77, C07H 2100, C12P 1934, C12Q 168, G01N 3002

Patent

active

049353420

ABSTRACT:
The method of this invention is applicable to rapid separation, isolation, and purification of DNA or RNA from biological samples. The DNA/RNA may be in double-stranded or single-stranded form. The method is particularly advantageous for resolving genetic DNA or RNA found in bacteria, virus, and mammalian cells, and for use with samples of human bodily fluids and tissues, including stool, sputum, urine, and blood samples. DNA or RNA can be separated effectively from interfering components, particularly proteins, biological pigments and mucopolysaccharides. The method of the present invention can utilize commercially available strong or weak anion exchanger materials with selected solutions of known ionic strength for adsorption and elution.

REFERENCES:
Osterman, L. A. (1984) Methods of Protein and Nucleic Acid Research (Springer-Verlag, Berlin, Germany) pp. 288-297.
Potter, A. A. et al. (1985) Cancer Letter 26, pp. 335-341.
Schleif, R. F. et al. (1981) Practical Methods in Molecular Biology (Springer-Verlag, Berlin, Germany) pp. 89-96.
Helms, C., et al. (1985) DNA, 4(1), pp. 39-49.
Schott, H. (1984) J. of Chromatography 284, pp. 409-431.
Colpan, M. et al. (1984) J. of Chromatography 296, pp. 339-353.
Ion Exchange Chromatography (published by Pharmacie Fine Chemicals in Sweden by Rahms; Lund, 1982) pp. 10-17.

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