Method of isolating and cryopreserving human white cells from wh

Details Method of isolating and cryopreserving human white cells from wh Method of isolating and cryopreserving human white cells from wh

1975-12-30

1977-01-25

Rosen, Sam

Textiles: fiber preparation

62 62, 424101, C12K 900, F25D 2500, A61K 3514

Patent

active

040049752

ABSTRACT:
A method of salvaging granulocytes, or white cells, from buffy coat of ceifuged whole blood is provided. The sequence of operations leading to the separation red cells, white cells, platelets and plasma is presented in a flow diagram of a bag system for the cryopreservation of leukocytes, with freezing of the white cells accomplished by the introduction of a combination of hydroxyethyl starch (HES), which functions as both a sedimenting agent and a cryoprotective agent, and dimethylsulfoxide (DMSO), a cryoprotective agent. A preferred combination is 4% HES with 5% DMSO. White cell separation is interfaced with collection methods for plasma and platelets so as to conserve all major cell types.

REFERENCES:
patent: 2910406 (1959-10-01), Novak
patent: 3170838 (1965-02-01), Archer
patent: 3328255 (1967-06-01), Ilg

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