Method of isolating acid-stable, biologically active proteins

Details Method of isolating acid-stable, biologically active proteins Method of isolating acid-stable, biologically active proteins

1992-03-19

1994-02-22

Wityshyn, Michael G.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530344, 530322, 530420, 530372, 530395, 530412, 530417, 530418, 530422, C07K 324

Patent

active

052888568

ABSTRACT:
A method of isolating cystine rich acid-stable, biologically active proteins comprising the steps of co-precipitation by acidification of said proteins together with at least one acid-sensitive protein; isolation of said proteins from the co-precipitate by its resuspension in aqueous solution and the subsequent recovery of the proteins from the supernatant.

REFERENCES:
patent: 4043990 (1977-08-01), Melachouris
patent: 4089848 (1978-05-01), Bell et al.
Odani, et al., J. Biochem., vol. 82, pp. 1513-1522 (1977).
Yoshikawa, et al., J. Biochem., vol. 87, pp. 619-627 (1986).
Hamelin, et al., Chem. Abs., vol. 93 entry #40799, (1980).
Brandao, et al., Chem. Abs., vol. 108, entry #220530m (1988).

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