Method of islet isolation using process control

Details Method of islet isolation using process control Method of islet isolation using process control

2006-05-16

2006-05-16

Lankford, Jr., Leon B. (Department: 1651)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

C435S286100, C435S366000, C435S375000, C435S378000, C435S379000, C435S380000, C435S381000

Reexamination Certificate

active

07045349

ABSTRACT:
Advanced Islet Separation Technology incorporates an automated method, automated control methodology, process control interface, and automated apparatus to separate (isolate) and process pancreatic islets in a tissue suspension in physiologic process solution, utilizing microprocessor controllers and computer control and software programming to interface and control the process temperature, process flowrate, percent hydrogen concentration, dissolved oxygen concentration, endotoxin concentration, dissolved nitric oxide concentration, nitric oxide synthase concentration, proteolytic enzyme activity, and pressure of the islet containing physiologic process solution, including real-time process data acquisition and recording of the process variables.

REFERENCES:
patent: 4868121 (1989-09-01), Scharp et al.
patent: 5079160 (1992-01-01), Lacy et al.
patent: 5273904 (1993-12-01), Langley
patent: 5322790 (1994-06-01), Scharp
patent: 5424209 (1995-06-01), Kearney
patent: 5612188 (1997-03-01), Shuler et al.
patent: 5834005 (1998-11-01), Usala
patent: 5837738 (1998-11-01), Williamson
patent: 5853976 (1998-12-01), Hesse
patent: 5879939 (1999-03-01), Gray
patent: 5919703 (1999-07-01), Mullen et al.
patent: 5919775 (1999-07-01), Amin
patent: 5952215 (1999-09-01), Dwulet et al.
Bond, M.D. and Van Wart, H.E., “Purification and Separation of Individual Collagenases ofClostridium histolyticumUsing Red Dye Ligand Chromatography,”Biochemistry1984; 23:3077-3085.
Bond, M.D. and Van Wart, H.E., “Characterization of the Individual Collagenases fromClostridium histolyticum, ” Biochemistry1984; 23:3085-3091.
Scharp, D.W., “Isolation and Transplantation of Islet Tissue,”World Journal of Surgery1984; 8:143-151.
Eckhardt, T., Jahr, H., Federlin, K. and Bretzel, R.G., “Endotoxin impairs the engraftment of rat islets transplanted beneath the kidney capsule of C57BL/6-mice,”Journal of Molecular Medicine1999; 77:123-125.
Jahr, H., Pfeiffer, G., Hering, B.J., Federlin, K., and Bretzel, R.G., “Endotoxin-mediated activation of cytokine production in human PBMCs by collagenase and Ficoll,”Journal of Molecular Medicine1999; 77:118-120.
Linetsky, E., Inverardi, L., Kenyon, N.S., Alejandro, R., and Ricordi, C., “Endotoxin Contamination of Reagents Used During Isolation and Purification of Human Pancreatic Islets,”Transplantation Proceedings1998; 30:345-346.
Smith, Jr., G.N., Brandt, K.D., and Hasty, K.A., “Procollagenase Is Reduced to Inactive Fragments upon Activation in the Presence of Doxycycline,”Annals New York Academy of Sciences1994; 732:436-438.
Vargas, F., Vives-Pi, M., Somoza, N., Armengol, P., Alcade, L., Marti, M., Costa, M., Serradell, L., Dominguea, O., Fernandez-Llamazares, J., Julian, J.F., Sanmarti, A., and Pujol-Borrell, R., “Endotoxin Contamination May Be Responsible for the Unexplained Failure of Human Pancreatic Islet Transplantation,”Transplantation1998; 65(5):722-727.
Scharp—World J. of Surgery—8:143-151 (1984).
Bond—Biochemistry—23:3085-3091 (1984).
Jahr—J. of Mol. Med. 77:(1):110-120 (1984).
Vargas—Trans. 65:(5):722-727 (1999).
Linetsky—Trans. Process—30:345-346 (1998).
Eckhardt J. of Mol Med (Berlin) 77(1):123-125 (1998).
Smith—Anals on N.Y. Acad Sci. 732:436-438 (1999).
Bond—Biochemistry 23:3077 (1984).

Affiliated with

Also associated with

Rating

Method of islet isolation using process control does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of islet isolation using process control, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of islet isolation using process control will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3543378