Chemistry: analytical and immunological testing – Including sample preparation
Patent
1997-07-18
1998-09-29
Pyon, Harold Y.
Chemistry: analytical and immunological testing
Including sample preparation
204193, 250372, 25045511, 250504R, 2504921, 356 51, 356 72, 364188, 364189, 36441326, 4221863, 435291, G01J 104
Patent
active
058145230
ABSTRACT:
A method of irradiating a biological specimen with ultraviolet, in particular a polynucleotide specimen selected from DNA or RNA, or optionally a protein. In the case where the specimen is DNA or RNA, or potentially proteins, the specimen is irradiated to cross-link the specimen to a substrate. In the case where the specimen is DNA, the specimen can also be irradiated to form thymine dimers. The method uses an apparatus which permits relatively precise control of the total ultraviolet dose received by the specimen, despite any changes of ultraviolet flux from the lamps which may occur from during any one experiment, or between a number of experiments. Thus, the method allows relatively highly reproducible results to be obtained.
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Sorge Joseph A.
Zimlich, Jr. William C.
Pyon Harold Y.
Stratagene
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