Method of irradiating biological specimens

Details Method of irradiating biological specimens Method of irradiating biological specimens

1991-04-17

1994-02-22

Warden, Robert J.

Chemistry: analytical and immunological testing

Including sample preparation

204193, 250372, 25045511, 250504R, 2504921, 356 51, 356 72, 364188, 364189, 36441326, 4221863, 435291, G01J 104

Patent

active

052886476

ABSTRACT:
A method of irradiating a biological specimen with ultraviolet, in particular a polynucleotide specimen selected from DNA or RNA, or optionally a protein. In the case where the specimen is DNA or RNA, or potentially proteins, the specimen is irradiated to cross-link the specimen to a substrate. In the case where the specimen is DNA, the specimen can also be irradiated to form thymine dimers. The method uses an apparatus which permits relatively precise control of the total ultraviolet dose received by the specimen, despite any changes of ultraviolet flux from the lamps which may occur from during any one experiment, or between a number of experiments. Thus, the method allows relatively highly reproducible results to be obtained.

REFERENCES:
patent: 3491234 (1970-01-01), Wiltrout
patent: 3852032 (1974-12-01), Urbach
patent: 4279254 (1981-07-01), Boschetti et al.
patent: 4400270 (1983-08-01), Hillman
patent: 5180611 (1993-01-01), Costello
Church et al, Proc. Nat'l. Acad. Sci. USA, vol. 81, pp. 1991-1995, Apr. 1984.

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