Method of ion exchange chromatography

Details Method of ion exchange chromatography Method of ion exchange chromatography

1977-10-26

1979-01-09

Adee, John

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

210 31C, 2603262, 26032627, 548344, 562443, 562445, 562447, 562516, 562554, B01D 1508

Patent

active

041337530

ABSTRACT:
The separation of constituents is carried out in such a way that a mixed amino acid sample is supplied to a separation column packed with a cation exchange resin and that, during the analysis of the single sample, five sorts of elutes of different compositions are supplied to the separation column in succession and by stages. The pH of the elute at the second stage is held higher than that of the elute at the first stage, and the pH's of the elutes at the third to fifth stages are held successively higher. However, the pH of the elute at the third stage is held lower than that of the elute at the second stage. On the other hand, the concentrations of counter ions contained in the elutes at the first to fifth stages are held successively higher inversely to the order in which the elutes are supplied. Notwithstanding that the pH of the elute at the third stage is lowered, the broadening of a component peak can be prevented by the increase of the counter ion concentration. Moreover, the analytical time is shortened as a whole.

REFERENCES:
patent: 3537821 (1970-11-01), Hrdina
patent: 3686118 (1972-08-01), Benson
patent: 4042327 (1977-08-01), Haney et al.

Affiliated with

Also associated with

Rating

Method of ion exchange chromatography does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of ion exchange chromatography, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of ion exchange chromatography will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1787640