Method of introducing spectinomycin resistance into plants

Chemistry: molecular biology and microbiology – Vector – per se

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

435 15, 4351723, 800205, C12N 1554, C12N 1582

Patent

active

052179028

ABSTRACT:
This invention relates to the discovery that the prokaryotic enzyme, aminoglycoside 3"-adenyltransferase (AGAT), in particular as encoded by a bacterial aadA gene, is useful as a selectable marker for transformed plants. The enzyme conveys resistance to spectinomycin and streptomycin. Such markers are particularly advantageous because they are non-lethal, provide rapid visual identification of transformed cells and permit selection in media containing either spectinomycin or streptomycin. In addition, AGAT may be used as a selectable marker which differentiates by enabling survival on selective media.

REFERENCES:
Chinault, et al (1986) Plasmid 15: 119-131.
Rogers, et al. in Methods in Enzymology 153, Academic Press, NY, 1987, pp. 253-277.
Hayford, M. B., et al., "Development of a Plant Transformation Selection System Based on Expression of Genes Encoding Gentamicin Acetyltransferases," Plant Physiol., 86:1216-1222 (1987).
Bevan, M. W., et al., "A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation," Nature, 304:184-187 (1983).
Rothstein, S. J., et al., "Promoter cassettes, antibiotic-resistance genes, and vectors for plant transformation," Gene, 53:153-161 (1987).
Eichholtz, D. A., et al., "Expression of Mouse Dihydrofolate Reductase Gene Confers Methotrexate Resistance in Transgenic Petunia Plants," Somatic Cell and Molecular Genetics, 13:67-76 (1987).
Jones, J. D. G., et al., "A dominant nuclear streptomycin resistance marker for plant cell transformation," Mol. Gen. Genet., 210:86-91 (1987).
Maliga, P., et al., "Improved expression of streptomycin resistance in plants due to a deletion in the streptomycin phosphotransferase coding sequence," Mol. Gen. Genet., 214:456-459 (1988).
Fromm, H., et al., "The molecular basis for rRNA-dependent spectinomycin resistance in Nicotiana chloroplasts," EMBO, 6:3233-3237 (1987).
Uchimiya, H., et al., "Co-expression and inheritance of foreign genes in transformants obtained by direct DNA transformation of tobacco protoplasts," Mol Gen Genet, 205:1-8 (1986).
Fraley, Robert T., et al., "Expression of bacterial genes in plant cells," Proc. Natl. Acad. Sci., USA, 80:4803-4807 (1983).
Ellis, R. John, "Molecular Chaperones: The Plant Connection," Science, 250:954-958 (16 Nov. 1990).
Weising, Kurt, et al., "Foreign Genes in Plants: Transfer, Structure, Expression, and Applications," Annu. Rev. Genet. 22:421-477 (1980).
Comai, L., et al., "Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate," Nature 317:741-744 (24 Oct. 1985).
Lund, Peter, et al., "Bacterial Chitinase Is Modified and Secreted in Transgenic Tobacco," Plant Physiol. 91:130-135 (1989).
Vaeck, Mark, et al., "Transgenic plants protected from insect attack," Nature 328:33-37 (2 Jul. 1987).
Amasino, Richard M., et al., "Changes in T-DNA methylation and expression are associated with phenotypic variation and plant regeneration in a crown gall tumor line," Mol. Gen. Genet. 197:437-446 (1984).
Hepburn, A. G., et al., "The role of Cytosine Methylation in the Control of Nopaline Synthase Gene Expression in a Plant Tumor," J. Mol. Appl. Genet. 2(3):315-329 (1983).
Rogers, S. G., et al., "Improved vectors for plant transformation: Expression cassette vectors and new selectable markers," in Methods in Enzymology, vol. 153, pp. 253-277, Academic Press, N.Y. (1987).
Potrykus, I., "Gene transfer to cereals: An assessment," Bio/Technology, 8:535-542 (Jun. 1990).
Svab, Z., et al., "Aminoglycoside-3"-adenyltransferase confers resistance to spectinomycin streptomycin in Nicotiana tabacum," Plant Molecular Biology, 14:197-205 (1990).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method of introducing spectinomycin resistance into plants does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of introducing spectinomycin resistance into plants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of introducing spectinomycin resistance into plants will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1933542

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.