Method of introducing organic molecules into target cells

Details Method of introducing organic molecules into target cells Method of introducing organic molecules into target cells

2005-03-22

2005-03-22

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

C435S325000, C435S372000

Reexamination Certificate

active

06869796

ABSTRACT:
The present invention relates to a method of introducing organic molecules carrying genetic information into isolated target cells, which comprises the step of passing a supernatant comprising said organic molecules through a fluidized collection of said target cells during an essentially constant flow. The fluidisation is provided by directing a flow of the supernatant so as to essentially counteract the gravitational force of the target cells, or alternatively a force applied thereon. Thus, the present method enables an efficient introduction of genetic information into the target cells.

REFERENCES:
patent: 5252216 (1993-10-01), Folena-Wasserman et al.
K. Krishna Mohan et al.,Conceptual Design of a Cellular Filter to Remove Trace Viral Contaminants in Blood, publ. Biotechnol. Prog., 1990, pp. 104-113.

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