Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Reexamination Certificate
2004-08-20
2010-12-07
Ketter, James S (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
C435S470000, C435S471000, C435S285200
Reexamination Certificate
active
07846731
ABSTRACT:
The present invention relates to a method of introducing nucleic acids into cells by electroporation, comprisingthe step (A) of loading nucleic acids to the surface of an electrode;the step (B) of adhering cells on the obtained nucleic acid-loaded electrode surface; andthe step (C) of applying electric pulses to the adhering cells. According to this method, not only efficient introduction of a gene into cells but also gene introduction at desirable timing and at desirable sites can be performed without damaging the adhering cells.
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Iwata Hiroo
Kato Koichi
Yamauchi Fumio
Ketter James S
Kyoto University
Wenderoth , Lind & Ponack, L.L.P.
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