Method of introducing nucelic acid

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S470000, C435S471000, C435S285200

Reexamination Certificate

active

07846731

ABSTRACT:
The present invention relates to a method of introducing nucleic acids into cells by electroporation, comprisingthe step (A) of loading nucleic acids to the surface of an electrode;the step (B) of adhering cells on the obtained nucleic acid-loaded electrode surface; andthe step (C) of applying electric pulses to the adhering cells. According to this method, not only efficient introduction of a gene into cells but also gene introduction at desirable timing and at desirable sites can be performed without damaging the adhering cells.

REFERENCES:
patent: 4441972 (1984-04-01), Pohl
patent: 5232856 (1993-08-01), Firth
T. Yoshikawa et al., “Transfection microarray of human mesenchymal stem cells and on-chip siRNA gene knockdown”, Journal of Controlled Release, vol. 96, pp. 227-232, 2004.
Pei, R. et al.; “Assembly of Alternating Polycation and DNA Multilayer Films by Electrostatic Layer-by-Layer Adsorption”; Biomacromolecules, American Chemical Society, US, vol. 2, No. 2, Mar. 8, 2001, pp. 463-468; XP002366101.
Raptis, L. et al.; “In Situ Electroporation of Large Numbers of Cells Using Minimal Volumes of Material”; Analytical Biochemistry, vol. 317, No. 1, Jun. 1, 2003, pp. 124-128; XP002404716.
Yamauchi F. et al.; “Spatially and Temporarily Controlled Gene Transfer by Electroporation into Adherent Cells on Plasmid DNA-loaded Electrodes”; Nucleic Acids Research 2004; vol. 32, No. 22 , Dec. 21, 2004; XP002404717.
Yamauchi F. et al.; “Layer-by-Layer Assembly of Poly(ethyleneimine) and plasmid DNA onto Transparent Indium-tin Oxide Electrodes for Temporally and Spatially Specific Gene Transfer”; Langmuir: The ACS Journal of Surfaces and Colloids; vol. 21, No. 18, Aug. 30, 2005; pp. 8360-8367; XP002404717.

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