Method of introducing exogenous genes into cultured cells or fer

Details Method of introducing exogenous genes into cultured cells or fer Method of introducing exogenous genes into cultured cells or fer

1997-06-26

1998-11-10

Elliott, George C.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

536 241, C07H 2104, C12N 1500

Patent

active

058342699

ABSTRACT:
There is provided a method of introducing exogenous genes into cultured cells or fertilized eggs, characterized in that each exogenous gene is introduced into chromosomal DNA in a cultured cell or fertilized egg under a condition where the exogenous gene is placed between insulators so as to ensure an insulated environment, such that the introduced exogenous gene will not be affected by any influence from adjacent genes in the cultured cell or fertilized egg.

REFERENCES:
patent: 5610053 (1997-03-01), Chung et al.
patent: 5650298 (1997-07-01), Bujard et al.
Chung et al. (Aug. 1993) A 5' element of the chicken beta-globin domain serves as an insulator in human erythroid cells and protects against position effect in Drosophila. Cell 74:505-514.

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