Method of introducing exogenous compounds into cells by electrop

Details Method of introducing exogenous compounds into cells by electrop Method of introducing exogenous compounds into cells by electrop

1996-09-23

1999-02-23

Weber, Jon P.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Cell membrane or cell surface is target

4351735, C12N 1300

Patent

active

058742689

ABSTRACT:
An electroporation apparatus for introducing exogenous material into cells is described herein. The apparatus comprises first a base member (15) configured for holding a cell support, the cell support having a top surface portion, with the top surface portion configured for carrying adherent cells. The apparatus further comprises an electrode carrier (25) operably associated with the base member, the electrode carrier having a bottom surface portion, a first electrode (30) connected to the electrode carrier, and a second electrode (35) also connected to the electrode carrier. The electrode carrier has a channel (40) formed therein, with the channel positioned between the first electrode and the second electrode, so that exogenous material may be introduced through the channel and into contact with the cells. Methods for introducing exogenous compounds into a cell and for visually detecting the location of binding events within a cell are also disclosed.

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