Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1997-05-21
1999-03-30
Guzo, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 71, 435 72, 435325, 435375, 435455, 536 231, 536 2372, 536 241, C12Q 168, C12Q 170, C12N 510, C07H 2102
Patent
active
058887270
ABSTRACT:
A method of inhibiting transport of nucleic acids between the nucleus and cytoplasm of a cell is disclosed. In one embodiment, this method comprises the step of exposing a cell to a sufficient amount of vesicular stomatitis virus M protein such that the transport of RNA and protein through the nuclear envelope of the cell is impeded. A chimeric RNA capable of nuclear export is also disclosed.
REFERENCES:
Orken et al., "Report and Recommendations of the Panel to Assess the NIH Investment in Research on Gene Therapy" Dec. 9, 1995.
Gorlich et al., Science, vol. 271, pp. 1513-1518, Mar. 15, 1996.
Verma et al., Nature, vol. 389, pp. 239-242, Sep. 18, 1997.
Her et al., Science, vol. 276, pp. 1845-1848, Jun. 20, 1997.
B.L. Black, et al., "The Role of Vesicular Stomatitis Virus Matrix Protein in Inhibition of Host-Directed Gene Expression is Genetically Separable from its Function in Virus Assembly," J. Virol. 67(8) :4814-4821, 1993.
Y. Cheng, et al., "Diverse Effects of the Guanine Nucleotide Exchange Factor RCC1 on RNA Transport," Science 267:1807-1810, 1995.
D.E. Crone and J.D. Keene, "Viral Transcription is Necessary and Sufficient for Vesicular Stomatitis Virus to Inhibit Maturation of Small Nuclear Ribonucleoproteins," J. Virol. 63(10) :4172-4180, 1989.
C. Featherstone, et al., "A Monoclonal Antibody Against the Nuclear Pore Complex Inhibits Nucleotcytoplasmic Transport of Protein and RNA In Vivo," J. Cell Biol. 107:1289-1297, 1988.
L.D. Fresco, et al., "Rapid Inhibition of Processing and Assembly of Small Nuclear Ribonucleoproteins after Infection with Vesicular Stomatitis Virus," Molec. Cell. Biol. 7(3) :1148-1155, 1987.
D. Gorlich and I.W. Mattaj, "Protein Kinesis: Nucleocytoplasmic Transport," Science 271:1513-1518, 1996.
C. Grimm, et al., "In vivo Selection of RNAs that Localize in the Nucleus," EMBO J. 16(4) :793-806, 1997.
C. Grimm, et al., "Selection and Nuclear Immobilization of exportable RNAs," Proc. Natl. Acad. Sci. USA 94:10122-10127, 1997.
E. Izaurralde and I.W. Mattaj, "RNA Export," Cell 81:153-159, 1995.
S.-Y. Paik, et al., "Inducible and Conditional Inhibition of Human Immunodeficiency Virus Proviral Expression by Vesicular Stomatitis Virus Matrix Protein," J. Virol. 69(6):3529-3537, 1995.
M.A. Powers, et al., "The Vertebrate GLFG Nucleoporin, Nup98, Is an Essential Component of Multiple RNA Export Pathways," J. Cell Biol. 136(2):241-250, 1997.
C. Tuerk and L. Gold, "Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase," Science 249:505-510, 1990.
Cheng Yan
Dahlberg James E.
Grimm Christian
Her Lu-Shiun
Lund Elsebet
Guzo David
Wisconsin Alumni Research Foundation
LandOfFree
Method of inhibition of nucleo-cytoplasmic transport by M protei does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of inhibition of nucleo-cytoplasmic transport by M protei, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of inhibition of nucleo-cytoplasmic transport by M protei will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1213418