Chemistry: analytical and immunological testing – Including sample preparation – Stabilizing or preserving
Patent
1986-05-08
1988-10-25
Marcus, Michael S.
Chemistry: analytical and immunological testing
Including sample preparation
Stabilizing or preserving
436 8, 436 18, 436811, 436826, 422 41, 435 2, G01N 3348, G01N 3366
Patent
active
047804190
ABSTRACT:
There is described a method of inhibiting glycolysis in blood samples by adding an acid to the blood sample to adjust pH of the blood to a level between 5.0 and 7.0.
Collected blood samples undergo glycolysis during storage due to progress of the reaction with glycolytic enzymes with a result that glucose is reduced and lactic acid and pyruvic acid formed by the glycolysis is increased. According to the method of the invention, the glycolysis as mentioned above can be inhibited simply by adjusting pH of the blood sample so that accurate determination of the above-mentioned components are feasible. The preferred acids are citric acid, malonic acid and maleic acid. Addition of citric acid to produce a level between 1.0 and 5.0 mg/ml (blood) is especially preferable. In the method of the invention, further addition of a fluoride compound, preferably NaF, improves the glycolysis-inhibiting action.
The method of the invention is preferably carried out by the use of blood-collection tubes in which an acid in an amount sufficient to adjust pH of the blood sample to a level between 5.0 and 7.0, and additionally, a fluoride compound are contained therein.
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Okuda Shyoji
Tanaka Kiko
Uchida Kazuo
Kummert Lynn M.
Marcus Michael S.
Terumo Corporation
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