Method of inhibiting blood coagulation in extracorporeal circula

Drug – bio-affecting and body treating compositions – Immunoglobulin – antiserum – antibody – or antibody fragment,... – Monoclonal antibody or fragment thereof

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4241301, 4241401, 4241411, 53038823, 53038824, 53038825, 604 4, A61K 39395, G07K 1528

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054378648

ABSTRACT:
This invention provides a method of inhibiting coagulation in extracorporeal circulation in a subject, comprising administration of a therapeutically effective amount of a monoclonal antibody which inhibits the ability of tissue factor to bind to factor VII/VIIa. The method prevents complex formation between tissue factor and factor VII/VIIa and thus inhibits coagulation of blood in extracorporeal procedures such as cardiopulmonary bypass and other shunt procedures. Anti-tissue factor monoclonal antibodies produced by hybridoma cell lines TFS-5G9 or TF9-6B4 may be used in the claimed methods.

REFERENCES:
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Carson, et al., Monoclonal Antibodies Against Bovine Tissue Factor, Which ock Interaction with Factor VII, Blood, 66:152,156, 1985.
Guha, et al. Affinity Purification of Human Tissue Factor: Interaction of Factor VII and Tissue Factor in Detergent-Micelles, Proc. Natl. Acad. Sci. USA, 83:299-302, 1986, Biochemistry.
Yanina T. Wachtfogel, et al.,; Formation of Cl.sub.s -Cl-Inhibitor, Kallikrein . . . Blood; vol. 73, No. 2; Feb., 19889; pp. 468-471.
Janos Kappelmayer, et al.; Upregulation of Mac-1 surface expression . . . Mosby/Journal Page 1 Sep. 11, 1992.
Yanina T. Wachtfogel, et al., Human Neutrophil Degranulation During . . . Blood, vol. 69, No. 1 Jan. 1987 pp. 324-330.
Jacek Musial, et al.; Loss of Fibrinogen Receptors from the Platelet . . . The Journal of Laboratory and Clinical Medicine; vol. 105, No. 4, pp. 514-522.
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L. H. Edmunds, Jr., et al.; Blood-Surface Interactions During Cardiopulmonary . . . Steinkopff Verlag Darmstadt; 1991; pp. 27-36.

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