Method of inducing the differentiation of stem cells into...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology

Reexamination Certificate

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C435S475000, C435S366000, C435S363000

Reexamination Certificate

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07727762

ABSTRACT:
For a method of inducing differentiation of cardiomyocytes from stem cells, a method is provided to induce efficiently and selectively differentiation of cardiomyocytes by such a method in which the stem cells are cultured to induce differentiation into cardiomyocytes in the presence of a substance that inhibits BMP signaling.

REFERENCES:
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Rajasingh, J et al, 2007, STAT-3-dependent mouse embryonic stem cell differentiation into cardiomyocytes, Circ. Res. 101:910-918.
Ruhnke et al, 2003, Stem Cells, 24:428-436.
Keller, GM, 2005, Genes and Development, 19:1129-1155.
Wang, G et al, 2005, Noggin and bFGF cooperate to maintain pluripotency of human embryoic stem cells in the absence of feeder layers, BBRC, 330:934-942.
Zhang et al, 2001, Am J Physiol Heart Circ Physiol, 280:H1782-1792.
Monzen et al., “Bone Morphogenetic Proteins Induce Cardiomyocyte Differentiation through the Mitogen-Activated Protein Kinase Kinase Kinase TAK1 and Cardiac Transcription Factors Csx/Nkx-2.5 and GATA-4.” Molecular and Cellular Biology, vol. 19, No. 10, pp. 7096-7105, Oct. 1999.
Kawai, et al. “Efficient Cardiomyogenic Differentiation of Embryonic Stem Cell by Fibroblast Growth Factor 2 and Bone Morphogenetic Protein 2.” Circulation Journal, vol. 68, pp. 691-702, Jul. 2004.
Pera, et al., “Regulation of Human Embryonic Stem Cell Differentiation by BMP-2 and its Antagonist Noggin”, Journal of Cell Science, vol. 117, p. 1269-1280, 2004.
Yuasa, et al., “Transient Inhibition of BMP Signaling by Noggin Induces Cardiomyocyte Differentation of Mouse Embryonic Stem Cells”, Nature Biotechnology, vol. 23, No. 5, p. 607-611, May 2005.
Monzen, et al. (May 14, 2001) “Smads, TAK1, and their Common Target ATF-2 Play a Critical Role in Cardiomyocyte Differentiation.” The Journal of Cell Biology 153(4): 687-698.

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