Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification
Reexamination Certificate
2011-06-28
2011-06-28
Vogel, Nancy (Department: 1636)
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
C435S455000, C435S468000, C435S471000, C435S325000, C435S410000, C435S254110
Reexamination Certificate
active
07968339
ABSTRACT:
The present invention relates to a method for increasing genetic recombination frequency in a genomic DNA and a method for inducing genome rearrangement. Specifically, according to the present invention there are provided: the method for increasing genetic recombination frequency in a cell in which genetic recombination takes place at any sites in the genome, comprising causing a restriction enzyme to be expressed in the cell, inducing transient activation of the restriction enzyme, and then introducing 2 or more double strand cleavages into any genomic DNA of the cell, so as to increase the genetic recombination frequency; the method for inducing genome rearrangement through the use of the above method; and cells each prepared through the use of the above 2 methods.
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Hirota Kouji
Ohta Kunihiro
Seo Hidetaka
Shibata Takehiko
Bozicevic Field & Francis LLP
Francis Carol L.
Riken
Vogel Nancy
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