Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-01-13
1999-01-26
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
536 221, 536 243, C12P 1934, C07H 2104
Patent
active
058637729
DESCRIPTION:
BRIEF SUMMARY
This application is a 341 of PCT/JP 06101246, filed May 10, 1996.
TECHNICAL FIELD
The present invention relates to a method of discriminating animal and plant individuals on the basis of a difference in DNA sequence, and more specifically relates to a method enabling individual discrimination by a polymerase chain reaction to be performed more efficiently as compared with a conventional method using a primer prepared on the basis of a known or optional base sequence; it relates to a new method of discriminating individual organisms which comprises conducting a polymerase chain reaction using specific DNA fragments (MI primer) as a primer, separating the amplified DNA fragments by acrylamide gel electrophoresis, staining each of the separated DNA fragments by silver staining, and discriminating the test individuals on the basis of a difference in the amplified DNA fragments.
TECHNICAL BACKGROUND
A method of discriminating animal and plant individuals on the basis of a difference in DNA sequence is internationally important from the standpoint of clarifying the genetic background of test individuals and protecting the rights of breeders and fosterers dealing with the same. As a method of individual discrimination on the basis of a difference in DNA sequence is used a PCR (polymerase chain reaction) method. In order to perform individual discrimination by the PCR method, a base sequence of a primer (short DNA fragments, indispensable for the PCR) is an important factor for the success of the discrimination.
Various primers prepared on the basis of known or optional base sequences have been developed, and methods of individual discrimination by using these known or optional base sequences as a primer for the PCR have been proposed (Skolnick, M. H. and R. B. Wallace, Genomics 2: 273-279 (1988); Williams, J. G. K. et al., Nucleic Acid Res. 18: 6531-6535 (1990)); however, according to conventional methods, it has been often difficult to clearly ascertain different DNA fragments exhibiting polymorphism among test individuals and improvements thereof have been demanded intensely.
In addition, as a method of discriminating test individuals on the basis of a difference in DNA fragments can be mentioned methods proposed in RFLP (Restriction Fragments Length Polymorphysms) (Tangsley, S. D. et al., Biotechnology 7: 257-264 (1989)), the official gazette of Japanese Laid-Open Patent Publication No. 62-500423 (1987) and the official gazette of Japanese Laid-Open Patent Publication No. 6-504427 (1994).
It is guessed that base sequences having a symmetrical structure of a mirror image are scattered among DNAs of organisms. That is, for example, it has been thought generally that the phylogenetic differentiation of plant species occurred along with the change of their genes (DNAs) in the process of evolution of them. It can be guessed by comparing the sequences of genes (DNAs) of different plant species with each other that the DNAs were recombined through domains having common base sequences in the phylogenetic differentiation. The present inventor has engaged in studies about the structural change of DNAs of plant species using rice plant as test materials and has found that when the recombination of DNAs (homologous recombination) is caused through a domain having a common base sequence, the recombinant DNA with a base sequence having a symmetrical structure of a mirror image (mirror image structure) in some cases (Kawata, M. et al., Theor. Appl. Genet. 90: 364-371 (1995)). According to the above, it can be guessed that DNAs of organisms including rice plant, corn and the like contain a base sequence having a symmetrical structure of the mirror image as traces of the recombinations of DNAs caused in the process of evolution of them.
Under these circumstances, taking the above prior arts into consideration, the present inventor has engaged in assiduous studies with a view to developing a primer useful for a method of individual discrimination by the PCR method, and has investigated the efficiency of a base sequence wi
REFERENCES:
patent: 5364759 (1994-11-01), Caskey et al.
patent: 5437975 (1995-08-01), McClelland et al.
David et al. Molecular Cloning of a Phosphatidylinositol-anchored Membrane Heparan Sulfate Proteoglycan from Human Lung Fibroblasts, J. Cell Biol., vol. 111(6), pp. 3165-3176, 1990.
Welch et al. Expression of a Glyceraldehyde 3-Phosphate Dehydrogenase Gene Specific to Mouse Spermatogenic Cells, Biol. Repr. vol. 46, pp. 869-878, 1992.
"Discrimination of Epidemic and Sporadic Isolates of Acinetobacter baumannii by Repetitive Element PCR-Mediated DNA Fingerprinting", A. C. Reboll, et al., Journal of Clinical Microbiology, vol. 32 No. 11 (1994), pp. 2635-2640.
Applied and Environmental Microbiology, vol. 60 No. 8(1994), M. R. Fries, et al., "Isolation, Characterization, and Distribution of Denitrifying Toluene Degraders from a Variety of Habitats", pp. 2802-2810.
Forage Crop Breeding and Seed Research Institute
Horlick Kenneth R.
Tung Joyce
LandOfFree
Method of individual discrimination by polymerase chain reaction does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of individual discrimination by polymerase chain reaction, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of individual discrimination by polymerase chain reaction will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1449704