Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1990-05-02
1992-01-07
Wax, Robert A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
4352523, 43525233, 4353201, 435 6, 536 26, 536 27, 536 28, 536 29, 935 79, C12Q 102, C12Q 168, C12N 120, C07H 1512
Patent
active
050791435
ABSTRACT:
A method for identifying compounds useful as antiparasitic drugs is disclosed. Deficient microorganisms are complemented with homologous metabolic enzyme genes. The metabolic enzyme is the drug target. In addition, recombinant DNA molecules comprising helminthic PFK and PEPCK genes are disclosed.
REFERENCES:
Hansen, E. J. and E. Juni, "Isolation of Mutants of Escherichia coli Lacking NAD- and NADP-Linked Malic Enzyme Activities", Biochem. Biophys. Res. Commun., 1975, 65(2)559-566.
Kemerer, V. F., et al., "Phosphofructokinase from Escherichia coli", Meth. Enzymol., 1975, 42:91-98.
Struhl, K., et al., "Functional Genetic Expression of Eukaryotic DNA in Escherichia coli", Proc. Natl. Acad. Sci. U.S.A., 1976, 73(5):1471-1475.
Ratzkin, B. and J. Carbon, "Functional Expression of Cloned Yeast DNA in Escherichia coli", Proc. Natl. Acad. Sci. U.S.A., 1977, 74(2):487-491.
Struhl, K. and R. W. Davis, "Production of a Functional Eukaryotic Enzyme in Escherichia coli: Cloning and Expression of the Yeast Structural Gene for Imidazole-Glycerolphosphate Dehydratase (his3)", Proc. Natl. Acad. Sci. U.S.A., 1977, 74(12):5255-5259.
Bach, M., et al., "Evidence for Transcriptional Regulation of Orotidine-5'-Phosphate Decarboxylase in Yeast by Hydridization of mRNA to the Yeast Structural Gene Cloned in Escherichia coli", Proc. Natl. Acad. Sci. U.S.A., 1979, 76(1):386-390.
Heinisch, J., "Isolation and Characterization of the Two Structural Genes Coding for Phosphofructokinase in Yeast", Mol. Gen. Genet., 1986, 202:75-82.
Rohrer, S. P., et al., "Purification and Characterization of Phosphoenolpyruvate Carboxykinase from the Parasitic Helminth Ascaris suum", J. Biol. Chem., 1986, 261(28):13049-13055.
Gehnrich, S. C., et al., "Liver (B-Type) Phosphofructokinase mRNA", J. Biol. Chem., 1988, 263(24):11755-11759.
Geary Timothy G.
Klein Ronald D.
DeLuca Mark
The Upjohn Company
Wax Robert A.
Zitomer Stephane W.
LandOfFree
Method of indentifying compounds useful as antiparasitic drugs does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method of indentifying compounds useful as antiparasitic drugs, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of indentifying compounds useful as antiparasitic drugs will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-821748