Method of increasing yield of t-PA in cell culture

Details Method of increasing yield of t-PA in cell culture Method of increasing yield of t-PA in cell culture

1988-01-26

1990-09-18

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435212, 435219, 43524023, C12N 964

Patent

active

049578634

ABSTRACT:
A method is disclosed for increasing the yield of t-PA in culture of mammalian cells comprising introducing antibodies to the t-PA of said cells into the cell culture nutrient medium, allowing the cells to grow, and then recovering t-PA from the t-PA-antibody complex thus found in the conditioned medium by exchanging antibody in the complex for t-PA antibody immobilized on an inert support.

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Einarsson, M., et al. (1985), Biochim. Biophys. Acta 830, 1-10.
Reagan, M. E., et al. (1985), Thromb. Res. 40, 1-9.
Kadouri and Bohak, Adv. Biotech, Proc. 5, Ed. Mizrahi and van Wezel, Alan R. Liss, Inc. 1985, pp. 275-299.
Jones and Griffen, Chem. Week, Apr. 1, 1987, pp. 38-41.
Arathoon and Birch, Science 232, 1390-1395 (1986).
Adamson and Schmidli, Can. J. Chem. Eng. 64, 531-539 (1986).

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