Method of increasing muscle mass with IGF-1 fusion polypeptides

Drug – bio-affecting and body treating compositions – Immunoglobulin – antiserum – antibody – or antibody fragment,... – Binds hormone or other secreted growth regulatory factor,...

Reexamination Certificate

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C514S012200, C530S350000, C530S399000

Reexamination Certificate

active

07837999

ABSTRACT:
A fusion protein comprising at least one IGF1 variant component and a fusion component (F), and, optionally, a signal sequence, exhibiting improved stability relative to the native IGF1 or IGF2 polypeptide. The fusion component (F) may be a multimerizing component, a targeting ligand, or another active or therapeutic compound. IGF1 variants were shown to have improved ability to induce skeletal muscle hypertrophy relative to native IGF1.

REFERENCES:
patent: WO/90/15142 (1990-12-01), None
patent: 0742228 (1996-11-01), None
Jansson et al., 1997, “Structural changes in insulin-like growth factor (IGF) I mutant proteins affecting binding kinetic rates to IGF binding protein 1 and IGF-I receptor.” Biochemistry Apr. 8;36(14):4108-17.
Jansson et al., 1998, “The insulin-like growth factor (IGF)binding protein 1 binding epitope on IGF-I probed by heteronuclear NMR spectroscopy and mutational analysis.” J Biol Chem. Sep. 18;273(38):24701-7.
Bayne et al., 1989, “The C region of human insulin-like growth factor (IGF) I is required for high affinity binding to the type 1 IGF receptor.” J Biol Chem. Jul. 5;264(19):11004-8.
Heding et al., 1996, “Biosensor measurement of the binding of insulin-like growth factors I and II and their analogues to the insulin-like growth factor-binding protein-3.” J Biol Chem. Jun. 14;271(24):13948-52.

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