Method of increasing expression of HLA, cell surface and TAA ant

Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Nitrogen containing other than solely as a nitrogen in an...

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514463, 514510, 549432, 554 63, 560174, 564223, A61K 3116, A61K 31335, A61K 3121

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056818602

ABSTRACT:
A composition for the upregulation of expression of cell antigens, without inducing shedding, which comprises a protein kinase C activator is provided by this invention. Further provided by this invention is a method of detecting and treating tumor cells comprising contacting tumor cells with an effective amount of a protein kinase C activator for the upregulation of expression of antigens of tumor cells, without inducing antigen shedding, and detecting the presence of said antigen or then further contacting said tumor cells with an effective amount of an antibody directed to said antigen.

REFERENCES:
Fisher, P.B. and Rowley, P.T., "Regulation of Growth, Differentiation and Antigen Expression in Human Tumor Cells by Recombinant Cytokines: Potential Applications for the Differentiation Therapy of Human Cancer," The Status of Differentiation Therapy of Cancer, vol. II, pp. 201-213, Raven Press, New York (1991) (Exhibit B).
Fuith, L.C., et al., J. Tumor Marker Oncology 6:85-89 (1991) (Exhibit C).
Greiner, et al., Cancer Res. 46:4984-4990 (1986) (Exhibit D).
Celada, A., et al., J. Immunol. 146:114-120 (1991) (Exhibit E).
Murray, J.L., et al., J. Biol. Response Mod. 9:556-563 (1990) (Exhibit F).
Giacomini, P., et al., J. Immunol. 133:1649-1655 (1984) (Exhibit G).
Greiner, J.W., et al., Cancer Res. 44:3208-3214 (1984) (Exhibit H).
Downey et al., "Phorbol Ester-Induced Actin Assemby", etc., The Journal of Cell Biology, vol. 116, No. 3 , Feb. 1992, pp. 695-706.
Reddy et al., "Evidence that Weak Promotion of Carcinogen-Initiated Cells Prevents their Progression to Malignancy", Carcinogenisis, vol. 11, No. 12, pp. 2123-2126, 1990.
Wender et al., "Analysis of the Phorbol Ester Pharmacophore on Protein Kinase C, etc." Proc. Natl. Acad. Sci. USA, vol. 83, pp. 4214-4218, Jun. 1986.
Leon et al., "Modulation of the Antigenic Phenotype of Human Breast Cancinomer Cells, etc." Cancer Immunol. Immunother. (1992) 35:315-324.

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