Method of in vitro differentiation of transplantable neural...

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of regulating cell metabolism or physiology

Reexamination Certificate

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C435S325000, C435S363000, C435S366000

Reexamination Certificate

active

06887706

ABSTRACT:
A method of differentiating embryonic stem cells into neural precursor cells is disclosed. In one embodiment the method comprises the steps of (a) obtaining an embryonic stem cell culture, (b) propagating the stem cells, (c) forming embryoid bodies from the stem cells, and (d) culturing the embryoid bodies in a medium containing an effective amount of fibroblast growth factor 2, wherein neural precursor cells will be generated and isolated.

REFERENCES:
patent: 5843780 (1998-12-01), Thomson et al.
patent: 6200806 (2001-03-01), Thomson et al.
patent: 6251669 (2001-06-01), Luskin
patent: 6468794 (2002-10-01), Uchida et al.
patent: 20020028510 (2002-03-01), Sanberg et al.
patent: 20020068045 (2002-06-01), Reubinoff et al.
patent: 20020114788 (2002-08-01), Isacson et al.
patent: WO 9747734 (1997-12-01), None
patent: WO 9920740 (1999-04-01), None
patent: WO 9920741 (1999-04-01), None
patent: WO 0151616 (2001-07-01), None
patent: WO 0181549 (2001-11-01), None
patent: WO 0183715 (2001-11-01), None
patent: WO 0188104 (2001-11-01), None
Brustle et al. In vitro-generated neural precursors participate in mammalian brain development. Proc. Natl. Acad. Sci. 94:14809-14814, 1997.*
Kawata et al. Neural rosette formation within in vitro spheroids of a clonal human tetracarcinoma cell line, PA-1/NR: Role of extracellular matrix components in the morphogenesis. Cancer Res. 51:2655-2669, 1991.*
Keystone Symposium on Pluripotent Stem Cells, Feb. 6, 2001 (Poster), Su-Chun Zhang, James A. Thomson, Ian David Duncan, Marius Wernig and Oliver Brustle.
M. K. Carpenter, et al., “Enrichment of Neurons and Neural Precursors from Human Embryonic Stem Cells,” Exp. Neurol. 172:383-397, 2001.

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