Method of improving the yield of heterologous proteins produced

Details Method of improving the yield of heterologous proteins produced Method of improving the yield of heterologous proteins produced

1990-09-28

1993-06-29

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435 691, C12N 1512

Patent

active

052234185

ABSTRACT:
A method is provided for improving the yield of the heterologous proteins sCD4 and derivatives of sCD4, produced by recombinant Streptomyces lividans by the addition of casamino acids to the nutrient medium in which the bacteria are cultured. Also provided by this invention is a method for improving the half-life of heterologous proteins in a cell free culture supernatant by the addition of casamino acids to the supernatant. Complex media are also provided.

REFERENCES:
patent: 4656132 (1987-04-01), Jean-Bassat et al.
patent: 4717666 (1988-01-01), Brawner et al.
patent: 4745056 (1988-05-01), Guterman et al.
patent: 4894334 (1990-01-01), Ben-Bassat et al.
Science, 238:1704-1707, Dec. 18, 1987, Smith et al. Blocking of HIV Infectivity by a Soluble, Secreted Form of the CD4 Antigen.
J. Ferment. Tech. 66:181-185, 1988, Fujimoto et al.
C. Walsh, Enzymatic Reaction Mechanisms, published 1979, W. H. Freeman & Co., see pp. 64-67.
Michael A. Whooley, John A. O'Callaghan and Aiden J. McLoughlin, "Effect of Substrate on the Regulation of Exoprotease Production By Pseudomonas aerugenosa ATCC 10145", Journal of General Microbiology, 129(4) 981-988 (1983).
E. Strydom et al., "Detection and Characterization of Extracellular Proteases in Butyrivibrio fibrisolvens H17C", Appl. Microbiol. Biotechnol, 24:214-217 (1986).
C. Wandersman, T. Andro and Y. Bertheau, "Extracellular Proteases In Erwinia chrysantemi, " Journal of General Microbiology, 132:899-906 (1986).
C. Ratcliffe et al., "Amylase and Protease Secretion by the Marine Bacterium Vibrio gazogenes", A. J. Biol. Sci., 35:457-67 (1982).
S. J. Copella and Prasad Dhurjati, ".alpha.-Factor Directed Expression of the Human Epidermal Growth Factor in Saccharomyces cerevisiae", Biotechnology and Bioengineering, 33:976-83 (1989).
G. K. Whitney, B. R. Glick and C. W. Robinson, "Induction of T4 DNA Ligase in a Recombinant Strain of E. Coli", Biotechnology and Bioengineering 33:991-998 (1989).
J. Arthos, et al., "Identification of the Residues in Human CD4 Critical for the Binding of HIV", Cell, vol. 57, 469-481, May 5, 1989.
C. LaLuce and R. Molinari, "Selection Of A Chemically Defined Medium For Submerged Cultivation of Streptomyces aureofaciens With High Extracellular Caseinolytic Activity", Biotechnology and Bioengineering, vol. XIX, 1863-1884 (1977).
Werner Aretz, Klaus P. Koller and Gunther Riess, "Proteolytic Enzymes from Recombinant Streptomyces lividans TK24", FEMS Microbiology Letters, 65:31-36 (1989).
Philippe Dehottay et al., "Cloning And Amplified Expression in Streptomyces lividans Of A Gene Encoding Extracellular .beta.-lactamase From Streptomyces albusG", Gene, 42:31-36 (1986).
J. R. Ludwig II, S. G. Oliver, and C. S. McLaughlin, "The Effect of Amino Acids on Growth and Phosphate Metabolism In A Prototrophic Yeast Strain", Biochemical and Biophysical Research Communications, vol. 79, No. 1, 16-23 (1977).
T. Erpicum et al., "Enzyme Production by Genetically Engineered Streptomyces Strains: Influence of Culture Conditions", Biotechnology and Bioengineering, 35:719-726 (1990).

Affiliated with

Also associated with

Rating

Method of improving the yield of heterologous proteins produced does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of improving the yield of heterologous proteins produced , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of improving the yield of heterologous proteins produced will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1754717