Method of improving the yield of heterologous protein produced b

Details Method of improving the yield of heterologous protein produced b Method of improving the yield of heterologous protein produced b

1984-03-28

1987-04-07

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4351723, 935 33, 935 38, 935111, C12P 2100, C12N 1500

Patent

active

046561323

ABSTRACT:
A method for improving the yield of heterologous protein such as IFN-.alpha., IFN-.beta., and IL-2, produced by recombinant bacteria by supplementing the nutrient medium in which the bacteria are grown with a water soluble alcohol and/or amino acid mixture during the terminal phase of the cultivation.

REFERENCES:
Ingram, 1976, J. Bact. v 125 (2) 670-678.
Lee et al, 1983, Proc Natl Acad Sci, v 80, 7496-7500.

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