Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Reexamination Certificate
2007-07-31
2007-07-31
Saidha, Tekchand (Department: 1652)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
C435S004000, C435S015000, C435S252300, C435S320100, C435S252330, C435S252500, C435S254100, C435S255100, C536S023200, C536S023700
Reexamination Certificate
active
11075398
ABSTRACT:
The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in the cell and incubating the cell with a suitable substrate to produce the biomass or product. This is conveniently done by expressing in the cell the soluble part (F1) of the membrane bound (F0F1type) H+ATPase or a portion of F1exhibiting ATPase activity. The organism from which the F1ATPase or portions thereof is derived, or in which the F1ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding F1or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the F1subunit β or a portion thereof and various combinations of the gene or portion with the genes encoding the other F1subunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing level of ATPase expression at which the conversion rate is optimized.
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Jensen Peter Ruhdal
Snoep Jacky Leendert
Westerhoff Hans Victor
Darby & Darby
Jensen Peter Ruhdal
Saidha Tekchand
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