Method of improving T cell receptors

Details Method of improving T cell receptors Method of improving T cell receptors

2008-10-07

2009-10-27

Gamett, Daniel C (Department: 1647)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C435S005000, C435S007200, C435S471000, C530S350000, C530S333000

Reexamination Certificate

active

07608410

ABSTRACT:
A method of increasing the affinity and/or decreasing the off-rate of a given TCR specific for a given target pMHC, comprising creating a plurality of TCRs having an α chain CDR2 sequence and/or a β chain CDR2 sequence different from the corresponding CDR2 sequence(s) of the given TCR but having the same α and β CDR1 and CDR3 sequences as the given TCR, determining the affinity and/or off-rate of members of said plurality of TCRs for the target pMHC, and selecting one or more members having at least a 10-fold greater affinity for the target pMHC than the given TCR and/or a 10-fold slower off-rate for the target pMHC than the given TCR.

REFERENCES:
patent: 6759243 (2004-07-01), Kranz et al.
patent: 2002/0048578 (2002-04-01), Waldmann
patent: 2002/0058253 (2002-05-01), Kranz
patent: 1 118 661 (2001-07-01), None
patent: WO 98/39482 (1998-09-01), None
Wu et al., (Nature, Aug 1, 2002; vol. 418, Iss.6897, p. 552-556).
Manning et al. (J. Exp. Med., vol. 189, No. 3, Feb. 1, 1999 461-470).
IPER and Written Opinion of the International Searching Authority for PCT/GB2005/001781, Nov. 2006.
Janeway et al. Immunobiology New York and London: Garland Science; c2001; Figure 5.18.
EP05741949 Applicant's response submitted on Sep. 13, 2007.
Churchill et al., “Mapping the energy of superantigenStaphylococcus enterotoxinC3 recognition of an alpha/beta T cell receptor using alanine scanning mutagenesis” Journal of Experimental Medicine, vol. 191, No. 5, Mar. 6, 2000.
Kieke et al., “High affinity T cell receptors from yeast display libraries block T cell activation by superantigens” Journal of Molecular Biology, London, GB, vol. 307, No. 5,Apr. 13, 2001, pp. 1305-1315.
Holler et al., “In vitro evolution of a T cell receptor with high affinity for peptide/MHC” Proceedings of the National Academy of Sciences of USA, National Academy of Science, Washington, DC, US, vol. 97, No. 10, May 9, 2000, pp. 5387-5392.
Chlewicki et al., “High-affinity, peptide-specific T cell receptors can be generated by mutations in CDR1, CDR2 or CDR3” Journal of Molecular Biology, vol. 346, No. 1, Feb. 11, 2005, pp. 223-239.

Affiliated with

Also associated with

Rating

Method of improving T cell receptors does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method of improving T cell receptors, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method of improving T cell receptors will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-4143503