Method of improving gene transfer efficiency into plant...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium

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C800S278000, C435S468000

Reexamination Certificate

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07902426

ABSTRACT:
A method for gene transfer by which higher efficiency for gene transfer than that by the conventionalAgrobacteriummethod may be attained simply and without injuring the tissue is disclosed. According to the method of the present invention, the efficiency of gene transfer into plant cells by a bacterium belonging to genusAgrobacteriumis promoted by accompanying heat treatment and centrifugation treatment of the plant cells or plant tissue.

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