Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Cell membrane or cell surface is target
Patent
1996-02-22
1998-08-18
Elliott, George C.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Cell membrane or cell surface is target
4351723, 4352852, C12N 1300, C12N 1563, C12M 304
Patent
active
057957550
ABSTRACT:
A method and apparatus for operating on living cells in order to transfer material to the interior of selectively targeted cells is disclosed. The operation may take place in a reaction chamber in which a group of cells are disposed, only some of which are desired to receive material which serves to transform or otherwise affect the cells. In one embodiment, certain of the cells in the reaction chamber are targeted by computerized image analysis of images of the cells as generated by confocal microscopy. The targeted cells are then transfected with genetic material using a computer controlled laser beam. The laser beam creates pores in the membranes of the targeted cells through which genetic material also present within the reaction chamber may pass. An electric field may also be employed to facilitate pore formation.
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Elliott George C.
McKelvey Terry A.
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