Method of immunossay including deactivation of endogenous alkali

Details Method of immunossay including deactivation of endogenous alkali Method of immunossay including deactivation of endogenous alkali

1990-03-01

1994-01-18

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 79, 435 792, 435 793, 435 794, 435 795, 435 21, 436501, 436518, C12Q 100, C12Q 142, G01N 3353, G01N 33566

Patent

active

052799352

ABSTRACT:
A method for immunoassay for a ligand is performed on a porous membrane with alkaline phosphatase as the label. The assay protocol includes a wash step with an organic acid to deactivate endogenous alkaline phosphatase. The invention includes a kit of materials for performing the assay.

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The Merck Index 10th (1983) pp. 330-331.
The Merck Index 10th Ed. (1983) Entry 3489.
Schlesinger, J. Biol. Chem. 1965 240:4284 The reversible dissociation of . . .
McComb et al., Alkaline Phosphatase, Plenum Publ., New York, New York, 1979, p. 413.
Holt et al., Proceedings of the Royal Society of London, B, 1958, 148, 481-494.

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