Method of immunological assaying human osteocalcin, reagent and

Details Method of immunological assaying human osteocalcin, reagent and Method of immunological assaying human osteocalcin, reagent and

1995-06-05

1997-10-28

Housel, James C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 792, 435 794, 435 795, 435975, 436512, 436518, 436531, 436534, 436547, 436548, 5303871, 5303881, 5303891, 5303911, 5303913, G01N 33535

Patent

active

056817070

ABSTRACT:
A method of immunologically assaying intact human osteocalcin in a human assay sample is provided by using an antibody having an epitope in a region of an amino acid sequence 1 to 20 on the N-terminal side of human osteocalcinand an antibody having an epitope in a region of an amino acid sequence 36 to 49 on the C-terminal side of human osteocalcin. Furthermore, a method of immunologically assaying the total amount of human intact osteocalcin in a human assay sample is provided. A reagent and a kit therefor are also described, as well as a monclonal antibody and a polyclonal antibody used for the assay a process for producing these antibodies; and a process for use of these antibodies.

REFERENCES:
patent: 4438208 (1984-03-01), Deftos et al.
patent: 5506111 (1996-04-01), Hosoda et al.

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