Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1979-07-25
1981-11-03
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435188, 435233, 424 12, 23230B, G01N 3354, C12N 996
Patent
active
042986869
ABSTRACT:
The invention relates to a novel enzymatic labelling agent for immunoenzymatic assays. The labelling agent is .DELTA..sub.5, 3-keto-steroid isomerase. The enzymatic activity bound to the antibody can be revealed advantageously by: (1) direct reading on the supernatent liquor or on the cake obtained by precipitation by means of a solvent, such as polyethylene glycol, with the use of soluble antibodies; (2) reading in solution after liberation of the enzyme from the antigen-antibody complex by a reducing agent, such as dithiotreitol; (3) immediate reading after filtration of a substrate solution on the insolubilized antibody-antigen-enzyme complex. The method can be used for immunoenzymatic assays automatically on substances present in very low concentrations, such as hypophysial hormones and plasmatic steroids.
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Jones, et al., "Steroids and Steroidases. IX." Canadian J. Chem., vol. 47, 1969) pp. 4459-4466.
Benson, et al., "Role of Reduced Glutothione in .DELTA..sup.s -3-Ketosteroid Isomerase Reaction of Liver", Biochem. Biophys. Res. Comm., vol. 69, No. 4 (1976) pp. 1073-1079.
Nicolas, et al. "New Amplification System for the Enzymic Determination of Microquantities of NAb (H): Applications and Perspectives", Chem. Abstracts, vol. 90, No. 1 p. 269 (1979) Abs. No. 2711q.
De Paulet Andre C.
Descomps Bernard
Nicolas Jean C.
Terouanne Beatrice
Institut National de la Sante et de la Recherche Medicale (INSER
Wiseman Thomas G.
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