Method of immobilizing proteinaceous substances

Details Method of immobilizing proteinaceous substances Method of immobilizing proteinaceous substances

1978-04-03

1979-09-25

Naff, David M.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

260112R, C07G 702

Patent

active

041690141

ABSTRACT:
Proteins such as enzymes are immobilized on a microporous member comprising a binder or matrix and finely divided filler particles dispersed throughout the binder. The proteins are coupled to the filler particles, and the microporous member has a relatively large surface area and a large number of available protein coupling sites. Enzymes coupled to the microporous member have a relatively high reaction efficiency when used to act on a substrate.

REFERENCES:
patent: 3766013 (1973-10-01), Forgione et al.
patent: 3791927 (1974-02-01), Forgione et al.
patent: 3862030 (1975-01-01), Goldberg
Wheeler, et al., Some Properties of Two Phosphatases Attached to Insoluble Cellulose Matrices, Biochim. Biophys. Acta, vol. 191, 1969, (pp. 187-189).
Thang, et al., Observations on the Activity of Enzymes after Filtration on (and through) a Nitrocellulose Membrane, Biochem. and Biophys., Res. Comm., vol. 31, No. 1, 1968 (pp. 1-8).

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