Method of immobilizing nucleic acid on a solid surface for use i

Details Method of immobilizing nucleic acid on a solid surface for use i Method of immobilizing nucleic acid on a solid surface for use i

1989-11-30

1993-06-01

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435810, 436518, 436530, 436808, 935 77, 935 78, C12Q 168

Patent

active

052158829

ABSTRACT:
Methods of immobilizing nucleic acid on a solid surface for us in nucleic acid hybridization assays is disclosed. The methods of the invention comprise reacting a modified nucleic acid strand comprising a variable portion and an anchor portion wherein the variable portion comprises a nucleotide sequence having a selected base sequence and the anchor portion comprises at least one nucleotide base modified with a primary amine function or nucleotide base equivalent having a primary amine function and reacting the modified nucleic acid strand with a free aldehyde group of the solid surface in the presence of a reducing agent to form complexes of the modified nucleic acid strand and at least a portion of the free aldehyde groups on the solid surface.

REFERENCES:
patent: 4423208 (1983-12-01), Grandics
patent: 4687808 (1987-08-01), Jarrett et al.
European Search Report, EP 90 31 3007, Mar. 28, 1991.
Wreschner, D. H. and Herzberg, M, Nucleic Acids Research 12: 1349-1359, (1984).
Gebeyechu et al., Nucleic Acids Research 15: 4513-4525, (1987).
Froehler, B. C., Tetrahedron Letters 27: 5575-5578 (1986).

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